Alafi Neuroimaging Laboratory

The mission of the Alafi Neuroimaging Laboratory is to provide access to state of the art imaging technology along with necessary expertise to scientists whose research requires whole slide scanning, image analysis, atomic force microscopy (AFM) imaging tools and/or FACS services. After completing mandatory training, the users are granted access to the facility and can reserve time on a particular instrument using a web-based calendar. Users who choose to work independently can rely on technical assistance and advice from the staff members. Alternatively, users can conduct their research in collaboration with a staff member. Users are billed monthly.  Rates are based on the instrument used and the time of day with substantial discounts for after-hours and weekend usage.

Service available to All entities, including for-profit organizations.

Priority service for Washington University only.

Additional information:

Orientation and training required. Please contact Kris Hyrc for details.​

• Manual and/or automatic scanning of standard slides stained for brightfield or fluorescence (DAPI, FITC, TRITC, Cy5, CY7) imaging.
• Visualizing the structure of molecules (e.g. proteins) and cells with the use of atomic force microscope.
• AFM measurements of surface properties (r.g. stiffness) of biological and non-biological materials.
• Quantitative image analysis.
• FACS based multi-parametric analysis of single cells and quantification of cell populations of interest.
• FACS based method for sorting a heterogeneous mixture of biological cells into multiple containers for downstream analysis such as cell culture or sequencing analysis.
• Training and assistance in using the instruments.
• Consultation on experimental design and execution.

• Hamamatsu NanoZoomer HT 2.0 slide scanning system.
• 3DHistech Pannoramic P250 Flash III digital slide scanner.
• Visiomorph-based image analysis system
• Asylum Research MFP-3D-BIO atomic force microscope.
• Beckman Coulter Cytoflex S analyzer.
• Beckman-Coulter SRT cell sorter.

Pricing is subject to core verification

Please check core website (https://hopecenter.wustl.edu/places/alafi-neuroimaging-core-charges/) or contact facility directly for pricing information.